Nitric oxide and prostacyclin play an important role in maintaining platelet-vessel homeostasis, especially in lung microcirculation, which, as shown in our studies, is disturbed in patients with acetylsalicylic acid- (ASA) induced asthma (AIA). Recently, researchers have shown great interest in the role of melatonin in the regulation of human physiological processes . Melatonin was shown to inhibit platelet aggregation induced by collagen, arachidonic acid, ADP or adrenaline . Melatonin also inhibits human platelet cyclo-oxygenase , and causes a relaxation of precontracted pulmonary arteries and veins . However, the role of melatonin in the pathogenesis of bronchial asthma remains unknown.
The goals of the present study were to examine the production of nitric oxide, prostacyclin and melatonin in AIA patients and to associate these findings with blood platelet function.
PATIENTS AND METHODS
Thirty-five patients with AIA, 27 patients with ASA-tolerant asthma (ATA) and 24 healthy volunteers were studied. Patients were examined when they were in stable clinical condition. All patients received typical therapy (oral theophylline or inhaled beta-adrenergic agonists, antihistamines, sodium cromoglycate and inhaled corticosteroids [daily dose of budesonide at most 800 |ig]).
Platelet aggregation activity was studied between 09:00 and 11:00. The change in light transmission of platelet-rich plasma was used to evaluate in vitro platelet aggregation and was measured in an aggregation analyzer AT-02 (SMC Komintern, St Petersburg, Russia) at 37°C according to the method of Born. You are always welcome to visit the my canadian pharmacy.com for your drugs to be delivered right on time and just as promised. This is the best place to shop for the treatment you need at this point.