Maintenance of Bovine Oocytes: RESULTS(3)

RESULTS(3)

The oocyte group classified as intermediate includes all of the oocytes morphologically classified as between GV stage and MII, and those that had degenerated.

Embryo Development after Antral Follicle Culture

The development of oocytes cultured for 24 h within intact antral follicles prior to maturation, fertilization, and culture was compared to that of control oocytes matured directly after aspiration. The results from this comparison are summarized in Table 3; 68.6% of control oocytes had developed to the 4+-cell stage 48 h after fertilization compared to 54.6% of oocytes after antral follicle culture. Buy Asthma Inhalers Online

Statistical analysis showed that there was a significant difference (x21 = 7.2, p < 0.01) in the cleavage rates between the control and follicle culture oocytes. The number of blas-tocyst-stage embryos produced after 8 days culture in vitro was 45 (32.8%) and 25 (48.4%) for the control and follicle cultured groups, respectively. Statistical analysis of these data showed a significant difference (x21 = 109.8, p < 0.001) between these two groups. There were no significant differences in terms of embryo quality based on total cell numbers, which were 144.6 ± 7.28 and 152.0 ± 8.44 in the control and treatment groups.
Fig6Maintenance of Bovine
FIG. 6. Example of an oocyte recovered after 3 days of antral follicle culture, showing expansion of the cumulus layer and detachment from the follicle wall. X400.

TABLE 3.

No. oocytesa No. 4 + -cell embryos (%)* No. blastocysts (%)** Total cell number
Control 186 131 (68.6 ± 8.6) 45 (32.8 ± 10.8) 144.6 ± 7.28
Follicle culture 94 51 (54.6 ± 13.9) 25 (48.4 ± 8.4) 152.0 ± 8.44

a 7 Replicates.
* p < 0.01, ** p < 0.001.

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