Australian Regenerative Medicine Institute

Intrauterine Infusion of High Doses of Pig Trophoblast: MATERIALS AND METHODS(1)

MATERIALS AND METHODS(1)Animal Management

The experiment was carried out on cyclic Meishan gilts (n = 15) derived from the experimental herd of Institut National de la Recherche Agronomique (INRA)-PRMD (Nouzilly, France). The number of estrous cycles preceding the experiment ranged from one to three. Estrus synchronization was performed with Alternogest (20 mg of Re-gumate daily for 18 days in the food; Hoechts-Roussel Vet., Romainville, France). Mean body weight and age were 61 kg (range 51-72) and 172 days (range 147-190), respectively. During the experiment, the gilts were kept in single pens on straw under natural lighting conditions. They received a standard pig diet twice daily and water ad libitum. The breeding, manipulations, surgery, and slaughter of the animals met approval of the INRA. They were performed in accordance with European regulations and with the Guiding Principles for the Care and Use of Research Animals promulgated by the Society for the Study of Reproduction. buy ampicillin

Surgical Procedures

Under general anesthesia, gilts were fitted surgically with indwelling jugular silastic catheters and uterine catheters 2-3 days before the beginning of the experiment. The catheter (inner diameter: 0.76 mm; Sigma Medical, Nan-terre, France) was inserted near the utero-tubal junction and pushed about 10 cm into each horn. The total length of the catheter was 70 cm. To protect the uterine and jugular catheters, the ends of the catheters were secured in small tissue bags stitched on the flank (jugular catheter) and on the back (uterine catheters). After surgery, the gilts received daily for four days 106 IU of benzylpenicillin and 800 mg of dihydrostreptomycin sulfate in the form of a 5-ml i.m. injection of Mixtencilline (Rhone-Merieux, Lyon, France).

Category: Trophoblast

Tags: Interferons, Intrauterine Infusion, Pig, Trophoblast