Identification of Specific Relaxin: RESULTS(3)

Immunohistochemistry results from sections of the mammary nipples are shown in Figure 5. Prominent labeling with the biotinylated relaxin was observed in epithelial cells of lactiferous duct, smooth muscle cells, and skin (Fig. 5A). Prominent labeling was also observed in sebaceous glands (Fig. 5A). Labeling of less intensity was observed in blood vessels (Fig. 5A). No signal was detected in sections incubated with unmodified porcine relaxin (Fig. 5B). Binding of the biotinylated relaxin in the mammary nipples was hormone-specific and saturable (Fig. 5, C and D).

Immunohistochemistry results from sections of term placenta are shown in Figure 6. Prominent labeling was observed in both amnion in chorionic plate and the large numbers of placental villi projecting into the lacuna system (Fig. 6, A-C). With the higher magnification of the chorionic plate, labeling was observed in amnion epithelial cells and smooth muscle cells surrounding blood vessels (Fig. 6B). Binding was also associated with stromal cells located within the chorionic plate (Fig. 6B). With the higher magnification of the placental villi, prominent labeling was observed in syncytiotrophoblast cells (Fig. 6C). Labeling of less intensity was observed in blood vessels within the villi (Fig. 6C). No signal was detected in sections incubated with unmodified porcine relaxin (Fig. 6D). Binding of the bio-tinylated relaxin in the term placenta was hormone-specific and saturable (Fig. 6, E and F). proventil inhaler
Fig5Identification of Specific
FIG. 5. Localization of relaxin-binding sites in the mammary nipples. Relaxin binding was localized in mammary nipples incubated with biotinylated relaxin (A) but not in mammary nipples incubated with unmodified porcine relaxin (B). Tissue sections incubated with biotinylated relaxin showed binding in the presence of a 2000-fold excess of human insulin (C) but not in the presence of a 2000-fold excess of porcine relaxin (D). dep, Lactiferous duct epithelial cells; sb, sebaceous glands; sm, smooth muscle; bv, blood vessels; sk, skin. Bar in A = 500 ^m; all panels are the same magnification.

Fig6Identification of Specific
FIG. 6. Localization of relaxin-binding sites in term placenta. Relaxin binding was localized in placentas incubated with biotinylated relaxin (A-C; B and C are a higher magnification of chorionic plate and villi in A, respectively) but not in placentas incubated with unmodified porcine relaxin (D). Tissue sections incubated with biotinylated relaxin showed binding in the presence of a 2000-fold excess of human insulin (E) but not in the presence of a 2000-fold excess of porcine relaxin (F). ae, Amnion epithelial cells; sy, syncytiotrophoblast cells; ch, chorionic plate; vi, villi; lac, lacuna; bv, blood vessels; st, stromal cells. Bar in A = 1000 ^m; D-F are the same magnification. Bar in B = 250 ^m; C is the same magnification.

This entry was posted in Relaxin and tagged Cells, Female, Human, Relaxin.