Identification of Specific Relaxin: RESULTS(2)

RESULTS(2)

Immunohistochemistry results from sections of the uterus are shown in Figure 3. Prominent labeling of both luminal and glandular epithelial cells and myometrial smooth muscle cells was observed in sections incubated with bio-tinylated relaxin (Fig. 3, A and B). Labeling of less intensity was also observed in blood vessels (Fig. 3A). With the higher magnification of the border between the endometrium and myometrium, binding appeared to be associated with individual cells in the stromal extracellular matrix (Fig. 3B). No signal was detected in sections incubated with unmodified porcine relaxin (Fig. 3C). Binding of the bio-tinylated relaxin in the uterus was hormone-specific and saturable (Fig. 3, D and E). canadian health & care mall

Immunohistochemistry results from sections of the mammary glands are shown in Figure 4. Prominent labeling of epithelial cells in both the lobular alveolar structures and lactiferous ducts as well as in blood vessels was observed in sections incubated with biotinylated relaxin (Fig. 4A). No signal was detected in sections incubated with unmodified porcine relaxin (Fig. 4B). Binding of biotinylated re-laxin in the mammary glands was hormone-specific and saturable (Fig. 4, C and D).
Fig3Identification of Specific
FIG. 3. Localization of relaxin-binding sites in the uterus. Relaxin binding was localized in uteri incubated with biotinylat-ed relaxin (A and B; B is a higher magnification of the border between the endometrium and myometrium in A) but not in uteri incubated with unmodified porcine relaxin (C). Tissue sections incubated with biotinylated relaxin showed binding in the presence of a 2000-fold excess of human insulin (D) but not in the presence of a 2000-fold excess of porcine relaxin (E). lep, Luminal epithelial cells; gep, glandular epithelial cells; ms, myometrial smooth muscle; bv, blood vessels; st, stromal cells. Bar in A = 1000 ^m; C-E are the same magnification. Bar in B = 100 ^m.

Fig4Identification of Specific
FIG. 4. Localization of relaxin-binding sites in the mammary glands. Relaxin binding was localized in mammary glands incubated with biotinylated relaxin (A) but not in mammary glands incubated with unmodified porcine relaxin (B). Tissue sections incubated with biotinylated relaxin showed binding in the presence of a 2000-fold excess of human insulin (C) but not in the presence of a 2000-fold excess of porcine relaxin (D). aep, Alveolar epithelial cells; dep, lactiferous duct epithelial cells; bv, blood vessels. Bar in A = 500 l^m; all panels are the same magnification.

This entry was posted in Relaxin and tagged Cells, Female, Human, Relaxin.