Identification of Specific Relaxin: RESULTS(1)

Samples of each tissue collected showed qualitatively similar results. Immunohistochemistry results from sections of cervix collected at hysterectomy are shown in Figure 1. Prominent labeling of luminal epithelial cells, smooth muscle cells, and cells associated with blood vessels was observed in sections incubated with biotinylated relaxin (Fig. 1A). No signal was detected in sections incubated with unmodified porcine relaxin (Fig. 1B). Binding of the bioti-nylated relaxin in the cervix was hormone-specific and saturable (Fig. 1, C and D).

Immunohistochemistry results from sections of the vagina are shown in Figure 2. Prominent labeling of luminal epithelial cells and both circular and longitudinal smooth muscle cells of the vagina was observed in sections incubated with biotinylated relaxin (Fig. 2, A-C). At the higher magnification, labeling was associated with smooth muscle surrounding blood vessels (Fig. 2, B and C). Relaxin binding also appeared to be associated with individual cells located within the stroma (Fig. 2, B and C). These are probably, at least in part, fibroblasts. No signal was detected in vaginal sections incubated with unmodified porcine relaxin (Fig. 2D). Binding of the biotinylated relaxin to the vagina was hormone-specific and saturable (Fig. 2, E and F). ventolin 100 mcg
Fig1Identification of Specific
FIG. 1. Localization of relaxin-binding sites in the cervix. Relaxin binding was localized in cervices incubated with biotinylated relaxin (A), but not in cervices incubated with unmodified porcine relaxin (B). Tissue sections incubated with biotinylated relaxin showed binding in the presence of a 2000-fold excess of human insulin (C), but not in the presence of a 2000-fold excess of porcine relaxin (D). ep, Epithelial cells; sm, smooth muscle; bv, blood vessels. Bar in A = 1000 ^m; all panels are the same magnification.

Fig2Identification of Specific
FIG. 2. Localization of relaxin-binding sites in the vagina. Relaxin binding was localized in vaginas incubated with biotinylated relaxin (A-C; B and C are a higher magnification of luminal and peripheral areas, respectively, of A), but not in vaginas incubated with unmodified porcine relaxin (D). Tissue sections incubated with biotinylated relaxin showed binding in the presence of a 2000-fold excess of human insulin (E), but not in the presence of a 2000-fold excess of porcine relaxin (F). ep, Epithelial cells; csm, circular smooth muscle; lsm, longitudinal smooth muscle; bv, blood vessels; st, stromal cells. Bar in A = 1000 ^m; D-F are the same magnification. Bar in B = 250 ^m; C is the same magnification.

This entry was posted in Relaxin and tagged Cells, Female, Human, Relaxin.