Australian Regenerative Medicine Institute

Identification of Specific Relaxin-Binding Cells in the Human Female(2)

The addition of synthetic human relaxin H2 to fetal membrane explants caused a dose-related increase in the expression of the genes, proteins, and enzyme activities of the matrix metalloproteinases interstitial collagenase (MMP-1), stromelysin (MMP-3), and gelatinase B (MMP-9).

A major step toward encouraging the view that relaxin has physiological and/or therapeutic roles in the human would be the demonstration that human tissues contain cells with specific relaxin-binding sites, i.e., putative relaxin receptors. There is limited evidence that human tissues contain relaxin-binding sites. 32P-Labeled human relaxin was reported to bind with specificity to both the decidua and cytotrophoblast layers in fetal membranes and to a human uterine cell line. Also, biotinylated human relaxin was reported to bind to the human cervix. None of these studies identified the cells to which relaxin bound. buy flovent inhaler

The objective of this study was to identify cells that contain specific and saturable binding sites for relaxin within several tissues that are potential targets for human relaxin, namely, cervix, vagina, uterus, mammary glands, mammary nipples, and placenta. To accomplish that objective, an in situ histochemical binding procedure recently developed by Min and Sherwood for identification of relax-in-binding cells in the pig was employed.

Category: Relaxin

Tags: Cells, Female, Human, Relaxin