Hormone-Specific Inhibitory Influence: DISCUSSION(8)

Increased FSH receptor-binding activity observed with N56dg-a:eFSH(b hybrid preparations as compared with the corresponding intact a:eFSH@ hybrid preparations was modest. This may help to explain the conflicting reports on the influence of haAsn52 oligosaccharide on recombinant hFSH binding to its receptor . As these recombinant hFSH preparations were expressed in three different cell lines, i.e., COS-7 monkey kidney cells , Chinese hamster ovary cells , and 293 human embryonic kidney cells , different glycosylation patterns likely resulted from cell-specific differences in oligosaccharide processing. buy ampicillin

Changes in immunoac-tivity due to altered glycosylation are generally ignored, yet may be significant . More importantly, the difference in magnitude of the negative inhibition, caused by increased length of the Asn56 oligosaccharide Man(al-6)Man antenna between eFSHp hybrids and those possessing either an eLHp or an eCGp subunit, suggested a different orientation of these naturally occurring FSH analogues in the FSH receptor from that observed for FSH.

This entry was posted in Hormone and tagged Equine Gonadotropins, Hormone, Oligosaccharide, Subunit Asn56.