G-CSFR Was Expressed on the Cell Surface of Trophoblast Cells in the Maternal Decidua
Immunohistochemical localization of G-CSFR in first-, second-, and third-trimester placental tissue is shown in Figure 1. Interstitial cytotrophoblast embedded in dense maternal connective tissue and decidual tissue distal and proximal to the materno-fetal interface in first- and second-trimester placental samples was positive for G-CSFR. The intensity of staining for G-CSFR on interstitial cytotropho-blast was weaker in the first trimester than in the second trimester (compare Fig. 1, a and c). Third-trimester interstitial trophoblast showed weak or absent staining for G-CSFR (Fig. 1e). Trophoblast in adjacent sections was identified by staining with antibody JMB2 (Fig. 1, b, d, and f). birth control yasmin
Adjacent sections treated with mouse IgG were negative (data not shown). Staining for G-CSFR was detected on the syncytial layer of first- and third-trimester placental tissues (Fig. 1, a and e) but not in second-trimester tissues (Fig. 1c), thus confirming our previous data. The staining for G-CSFR was particularly intense on the apical surface of these cells (Fig. 1, a and e), with weak or absent staining in the underlying cytotrophoblast of first-trimester chorionic villi (Fig. 1, a and e). In contrast, G-CSFR staining on first- (Fig. 1a) and second- (Fig. 1, c and g) trimester, cy-tokeratin-positive interstitial trophoblast was diffuse. High-power magnification of sections of second-trimester decidual tissue showing interstitial trophoblast positive for G-CSFR and cytokeratin is shown in Figure 1, g and h, respectively. This staining pattern was similar in first-trimester interstitial trophoblast. Adjacent sections treated with mouse IgG were negative (data not shown).
FIG. 1. Immunohistochemical detection by APAAP (pink staining) of G-CSFR using mAb LM832 (left panels) and cytokeratin using mAb JMB2 (right panels), at the materno-fetal interface in decidua in first- (a, b), second- (c, d, g, h), and third- (e, f) trimester tissues. Staining for G-CSFR was present on the cell surface of interstitial trophoblast cells (black arrows) in first- (a) and second- (c) trimester decidual tissues. Third-trimester interstitial trophoblast was negative for G-CSFR. G-CSFR-positive syncytio-trophoblast (white arrows) was present in first- and third-trimester chorionic villus (a, e). Second-trimester syncytiotrophoblast (c) was negative for G-CSFR. A high magnification of second-trimester tissue shows diffuse staining for G-CSFR in interstitial trophoblast (black arrow) (g). Trophoblast in adjacent sections was positive for cytokeratin (right panels). a-f X160, g and h X240 (reproduced at 62%).
FIG. 2. Immunohistochemical detection by APAAP (pink staining) ofG-CSFR using mAb LM832 (a, cytokeratin using mAb JMB2 (b), or mouse IgG (c), in fetal membrane rolls at term. Diffuse staining for G-CSFR was present in chorionic cytotrophoblast. Chorionic cytotrophoblast in adjacent sections was positive for cytokeratin. There was no staining in the negative control (c). T, Trophoblast layer; a, amnion; c, chorion (X208) (reproduced at 62%).