Australian Regenerative Medicine Institute

Effects of Photoperiod and Age: MATERIALS AND METHODS(2)

MATERIALS AND METHODS(2)

Trial 3

Male turkeys (RBC-3 line) 10 wk of age were used and treated as described for trial 1. Two weeks prior to serial bleeding, males were chosen at random and caged individually in floor pens (60 X 82 cm) with wood shavings as litter. Serial bleeding was conducted on birds at 13, 23, and 35 wk of age under either LD or SD lighting. At each age, 8 birds, 4 given each lighting treatment, were serially bled. Blood samples were withdrawn from the jugular vein through a cannula system described by Chapman et al.. Briefly, the cannulas (medical-grade silicon tubing) were inserted percutaneously about 10 cm into the right jugular vein and affixed in the skin of the neck. The cannula was fastened to a swivel system and then passed through a hole in the room wall to the outside of the animal room, where blood sampling was performed. buy ventolin inhalers

This system allowed the individual birds free range and access to feed and water within the individual cages, as well as exposure to the experimental lighting program without interruption during blood sampling. Blood withdrawal operations were performed outside the room housing the animals so as not to disturb the birds or alter their behavior. Blood samples (1.0 ml each time) were collected every 10 min for 6 h, then every 5 min for an additional 3 h. Serial bleeding started at the beginning of the photophase for both the SD and LD photoperiods. Saline (0.7% NaCl plus 0.5% sodium citrate), equivalent in volume to the blood sample volume, was infused through the cannula after each sample. After serial bleeding, the birds were killed. The testes were then removed and weighed, and a portion of the right testis was fixed, embedded, sectioned, and stained for histological examination as previously described.

Category: Semen Production

Tags: Luteinizing Hormone, Male, Semen Production, Testosterone