Australian Regenerative Medicine Institute

Effects of Photoperiod and Age: MATERIALS AND METHODS(1)

Trial 1

Male turkeys 10 wk of age (RBC-3 line, Ohio State University; ) were housed in light-tight rooms and given one of two lighting treatments: either LL (long-long), in which the turkeys (n = 13) were maintained under a long-day (LD) photoperiod of 16L:8D throughout the trial; or SL (short-long), in which the turkeys (n = 13) were maintained under a photoperiod of 6L:18D (SD) until 29 wk of age, when they were switched to LD. Food and water were available ad libitum. Blood samples (1.5 ml) were collected weekly from 10 to 29 wk of age from the jugular vein, then daily for 2 days after the switch to LD, when the trial was terminated. Semen collections were attempted weekly on all birds beginning at 20 wk of age. Semen production was measured gravimetrically. Body weights were recorded weekly. buy flovent inhaler

Trial 2

To confirm the results from trial 1, male turkeys (RBC-3 line) 12 wk of age were used and treated as described for trial 1. The LL group had 8 birds; the SL group had 9 birds. After switching the SL group to LD at 29 wk of age, daily blood sampling was conducted for 5 days, and sampling then was performed weekly until 35 wk of age, when the trial was terminated. Semen collections were attempted from all birds weekly beginning at 18 wk of age. Semen production was determined gravimetrically. Body weights were recorded weekly. When the trial was terminated, the birds were killed and testes weights were determined.

Category: Semen Production

Tags: Luteinizing Hormone, Male, Semen Production, Testosterone