Effects of hypoxia on myoglobin and antioxidant enzymes in rat myocardium (part 4)

in rat myocardium (part 4)

Myocardial antioxidants and malondialdehyde determination: Rat hearts were removed after death as described above. Left and right ventricles (about 0.15 g) were homogenized in 10 volumes (1.5 mL) of normal saline. The homogenate was centrifuged at 20,000 g for 30 mins at 4°C, and the supernatant was assayed by spectrophotometry. Superoxide dismutase (SOD) activity was determined by inhibition of py-rogallol antioxidation . A unit of the enzyme is generally defined as the amount of enzyme that inhibits the reaction by 50%. Get most advantageous deals offered to you by the pharmacy you are going to appreciate soon after you become its customer: you now can get your Symbicort Price any time of the day or night with very fast delivery and quality guarantees.

Catalase (CAT) activity was assayed according to the method described by Aebi . The decomposition of H2O2 can be followed directly by the decrease in absorbance at 240 nm. The difference in absorbance per unit time is a measure of CAT activity. The following rate constant of a first-order reaction (k) was used as the definition of CAT units: 

where At is the time interval, Ao is the absorbance at time 0, At is the absorbance at time t and f is the dilution factor. Malon-dialdehyde (MDA) was measured according to the method described by Ohkawa et al .

Statistical analysis: All data are expressed as mean + SE. Differences between the groups were compared by Student’s t test. Statistical significance was accepted as P<0.05.

This entry was posted in Myocardium and tagged Antioxidant enzymes, Intermittent hypoxia, Myoglobin.