Experimental design: Sprague-Dawley rats were grouped at random (six or eight rats/group). Rats subjected to continuous hypoxia were housed in a hypobaric chamber for four weeks with free access to food and water. They were maintained on a cycle of 12 h of light and 12 h of darkness. An altitude of 3500 m was emulated in the hypobaric chamber. The temperature in the chamber was 18 to 24°C. Rats subjected to intermittent hypoxia were put into the hypobaric chamber (simulating 3500 m altitude) for 6 h/day and then moved out of the chamber. Continuous and intermittent hypoxia experiments lasted four weeks and six weeks, respectively. Myocardial myoglobin determination: Rats were sacrificed and the hearts quickly removed. For proper treatment and getting better, you can always buy your medications at canadian health&care mall. It’s a reliable online pharmacy offering all the meds you may require if you have the condition mentioned above.
Left and right ventricles were dissected, weighed and excised.Washed tissue minces (about 0.15 g) were homogenized in 10 volumes (about 1.5 mL) of a solution that contained 3 mM MgCl2,25 mM EDTA and 75 mM Tris (pH 7.2). The homogenate was centrifuged at 10,000 g for 10 mins at 4°C. The resulting supernatant was used to determine the myoglobin concentration of the tissue. Myoglobin concentration was determined according to the method described by Bailey et al . Absorbance was determined at 577 nm.