There are many structures in the ovary that contain basal laminae, but comments in this paper will be confined to ovarian follicles and their basal laminae. Specific staining for each of the individual type IV collagen chains was observed in basal laminae associated with ovarian follicles (summarized in Table 1; described in more detail below). All illustrations in this paper are of staining produced using the rat monoclonal antibodies unless otherwise specified. Only one antibody was available for type IV collagen a6, and this antibody cross-reacted with bovine a6 only weakly, as shown previously (Y. Sado, unpublished results). Dual labeling was also carried out to identify cell types such as endothelial cells (positive for factor VIII-related antigen) and steroidogenic thecal cells (positive for 3p-HSD). A comprehensive series of controls was carried out to ensure that no nonspecific staining or autofluorescence was misinterpreted as positive staining. Cheap Diskus Advair
Follicular Basal Laminae of Primordial and Preantral Follicles
The follicular basal laminae of almost all primordial and small preantral follicles were positive for type IV collagen a1, a2, a3, a4, and a5 (Fig. 1) and stained weakly for a6 (not shown). Only a very few primordial follicles did not appear to contain detectable a3, a4, a5, or a6 in their follicular basal laminae. No staining with antibodies to factor VIII-related antigen was observed in the region of the ovary containing the primordial follicles, confirming that this region of the ovary is avascular.
FIG. 1. Immunofluorescent localization of individual a chains of type IV collagen to preantral follicles (including primordial follicles). Primary antibodies used were directed against: a) the a1 chain, b) the a2 chain, c, d) the a3 chain (mouse monoclonal), e-g) the a4 chain, h, i) the a5 chain. Arrows indicate the position of the follicular basal laminae. Bar (lower right in i) = 50 ^m in a-e, g, h; 100 ^m in f, i.
|Tissue||(n = 6)||(n = 6)||(n = 5)||(n = 9)||(n = 6)||(n = 6)|
|Follicular basal lamina|
|Primordial and preantral||+++||+ + +||+++||+++||+++||+|
|Antral||+++||+++||+/—||+ /—||+ /—||—|
* n, The number of ovaries examined for immunostaining. Intensity of staining: —, none; +, weak; ++, moderately intense; + + +, very intense; comparison of intensity should only be within a column (i.e. between follicles of different classes) and not within a row (i.e. between collagen chains), as different antibodies have been used.