These were conjugated either with different fluo-rophores or with one conjugated to biotin-SP to allow further amplification by incubation with streptavidin conjugated to either Cy3 or FITC. Control sections for dual staining were as follows: the relevant polyclonal primary antibody and anti-mouse secondary antibody; the relevant mouse monoclonal primary antibody and anti-rabbit secondary antibody; the relevant rat monoclonal primary antibody and anti-rabbit secondary antibody; normal rabbit serum primary antibody and anti-rabbit secondary antibody; normal mouse serum primary antibody and antimouse secondary antibody; normal rat serum primary antibody and anti-rat secondary antibody; both normal rabbit serum and normal mouse serum primary antibodies and both anti-rabbit and anti-mouse secondary antibodies; both normal rabbit serum and normal rat serum primary antibodies and both anti-rabbit and anti-rat secondary antibodies. antibiotics levaquin
Observations and Photography
Immunostaining was visualized using either an Olympus (Tokyo, Japan) AX70 fluorescent microscope, with the selective NG filter (575-615 emission) for detecting the Cy3 fluorophore and an NIB filter (515-545 emission) for DTAF fluorophore, or an Olympus Vanox AHBT3 fluorescence microscope using the IB filter (490 emission) to excite the DTAF fluorophore and the G filter (546 emission) to excite the Cy3 fluorophore.