Distribution of the «1 to «6 Chains of Type IV Collagen: MATERIALS AND METHODS(5)

Sections were then washed (four times, 5 min each in hPBS) and incubated with the appropriate fluorophore-con-jugated secondary antibodies for 2 h. To achieve further amplification of the immunostaining, in some instances sections were instead incubated with secondary antisera conjugated with biotin-SP (diluted with antibody diluent) for 2 h; they were then washed (four times, 10 min each in hPBS) and further incubated with Cy3- or FITC-conjugated streptavidin (1:100 diluted in antibody diluent) for 1 h. All incubations were carried out at room temperature in a humidified environment. After final washing (four times, 10 min each in hPBS), sections were mounted with buffered glycerol (0.167 M Na2CO3 in 67% glycerol, pH 8.6). antibiotic levaquin

The protocol for dual labeling was essentially the same as for single labeling as described above, except that the sections were incubated concurrently with two primary antibodies of different species and were subsequently incubated concurrently with the two appropriate secondary antisera to enable discrimination between the primary antibodies.

This entry was posted in Follicles and tagged Bovine, Chains, Collagen, Follicles.