Category Archives: Trophoblast

Intrauterine Infusion of High Doses of Pig Trophoblast: DISCUSSION(3)

A previous experiment showed that total protein extract from pig conceptuses did not affect CL activity when injected in utero to cyclic gilts during Days 12-15. In this experiment, 4-mg doses of secretory proteins, collected from supematants of conceptus cultures … Continue reading

Intrauterine Infusion of High Doses of Pig Trophoblast: DISCUSSION(2)

In comparison, various experiments have been carried out in cyclic ewes, showing that total conceptus secretory proteins and/or purified IFN-t, or recombinant IFN were all able to significantly prolong luteal activity although to varying extents. Even at doses of IFN-т … Continue reading

Intrauterine Infusion of High Doses of Pig Trophoblast: DISCUSSION(1)

The present data show the lack of a significant effect of repeated injections of a mixture of IFN-7 and IFN-S on two parameters indicative of the CL activity in cyclic gilts, i.e., the day of the mid-decrease of progesterone (Figs. … Continue reading

Intrauterine Infusion of High Doses of Pig Trophoblast: RESULTS(3)

Ovulation All females of both groups had ovulated after return to estrus on the basis of progesterone levels in plasma and the examination of ovaries after slaughter. The mean ovulation rate after return to estrus was 13 ± 1.1 in … Continue reading

Intrauterine Infusion of High Doses of Pig Trophoblast: RESULTS(2)

Progesterone Plasma Levels The progesterone levels in the plasma of each individual gilt are presented in Figure 1A for controls and in Figure 2A for IFN-treated gilts. In both groups, plasma progesterone levels dropped between Day 14 and Day 16 … Continue reading

Intrauterine Infusion of High Doses of Pig Trophoblast: RESULTS(1)

In order to test the effect of porcine trophoblastic IFNs on the luteal cycle, two groups of cyclic Meishan gilts can-nulated in both uterine horns received injections of either a placebo (control group, n = 8) or increasing doses of … Continue reading

Intrauterine Infusion of High Doses of Pig Trophoblast: MATERIALS AND METHODS(5)

Examination at Slaughter The uterus and ovaries were examined after slaughter 15 days after the end of estrus. The number of ovulations was recorded at slaughter by dissection of all CL. The number of ovulations in the previous cycle (during … Continue reading

Intrauterine Infusion of High Doses of Pig Trophoblast: MATERIALS AND METHODS(4)

Blood Collection and Progesterone Assay Starting on Day 11 of the estrous cycle, blood samples (5 ml) were taken twice daily (0930 and 1730 h) until the next expected estrus so that progesterone could be analyzed. After each blood collection, … Continue reading

Intrauterine Infusion of High Doses of Pig Trophoblast: MATERIALS AND METHODS(3)

On the basis of previous results on IFN gene expression in the trophectoderm, we assumed that in pregnant uteri the relative proportion of each IFN species was unchanged from Day 11 to Day 14. A unique mixture was then prepared … Continue reading

Intrauterine Infusion of High Doses of Pig Trophoblast: MATERIALS AND METHODS(2)

Sources and Assay of IFNs Pure recombinant porcine IFN-7, produced in Escherichia coli, was kindly provided by Ciba-Geigy (Basel, Switzerland). Its specific antiviral activity was 1.2-1.5 X 105 U/mg protein. A specific sandwich-type ELISA was set up in our laboratory … Continue reading

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