Category Archives: Relaxin - Part 2

Identification of Specific Relaxin: MATERIALS AND METHODS(5)

After incubation, tissue slides were rinsed for 2 h with five changes of wash buffer (1% BSA fraction V, 0.2% fish gelatin, and 2 mM NaN3 in PBS). The tissues were then fixed for 30 min with 3% glutaraldehyde in … Continue reading

Identification of Specific Relaxin: MATERIALS AND METHODS(4)

Immunohistochemical Localization of Biotinylated Relaxin Immunohistochemical localization of biotinylated relaxin followed the procedure of Min and Sherwood. In brief, the tissue slides were brought to room temperature, and subsequent immunohistochemical procedures were performed at room temperature. Tissue slides were incubated … Continue reading

Identification of Specific Relaxin: MATERIALS AND METHODS(3)

The reaction mixture was stirred at room temperature for 4 h, and the reaction was stopped by the addition of acetic acid until a 1 M acetic acid solution was obtained. The contents of the reaction mixture were separated from … Continue reading

Identification of Specific Relaxin: MATERIALS AND METHODS(2)

Each tissue sample was cut into slices (0.5-3 cm3) and individually placed in peel-A-way plastic embedding molds (Polysciences, Inc., Warrington, PA). The tissues were frozen with Tissue-Tek O.C.T. embedding compound (Miles Scientific, Elkhart, IN) in liquid nitrogen and stored at … Continue reading

Identification of Specific Relaxin: MATERIALS AND METHODS(1)

Tissue Collection and Processing for Immunohistochemistry Human tissue samples were obtained immediately after clinical procedures. Uterine cervix, vagina, and uterus were obtained from hysterectomy specimens from six patients ranging from 29 to 51 yr of age. Five of the patients … Continue reading

Identification of Specific Relaxin-Binding Cells in the Human Female(2)

The addition of synthetic human relaxin H2 to fetal membrane explants caused a dose-related increase in the expression of the genes, proteins, and enzyme activities of the matrix metalloproteinases interstitial collagenase (MMP-1), stromelysin (MMP-3), and gelatinase B (MMP-9).

Identification of Specific Relaxin-Binding Cells in the Human Female(1)

In the human, there are two nonallelic genes for relaxin, designated H1 and H2. The corpus luteum is the primary source of circulating relaxin in women, and it produces only H2 relaxin. Plasma relaxin levels are elevated throughout gestation, with … Continue reading

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