Australian Regenerative Medicine Institute

Category: Platelet-Derived

Essential Role of Platelet-Derived: DISCUSSION(5)

However, it is of note that IPY development is strictly regulated in such a way that columnar epithelium and squamous epithelium face the chorion layer and labyrinthine layer, respectively. Since PDGFRa is expressed in both types of epithelium, PDGFRa alone could not establish such an organization, suggesting that another molecule is required for correct placement…

Essential Role of Platelet-Derived: DISCUSSION(4)

The extension of calbindin-D9k-positive villous endoderm into the periplacental region in the Pdgfraph/ Pdgfraph placenta may reflect invaginating movement of the visceral endoderm, which cannot develop into actual extension and invagination in the absence of PDGFRa. Under normal conditions, this process together with the signal from PDGFRa may lead to rapid proliferation and invagination of…

Essential Role of Platelet-Derived: DISCUSSION(3)

The yolk sac of the Pdgfraph/Pdgfraph embryo with a C57BL/6 genetic background was reported to be hypertrophic as compared with the wild-type embryo, suggesting a role of PDGFRot in the early process of yolk sac formation. However, Pdgfraph/Pdgfraph mutants with other genetic backgrounds, such as our outbred lines, continued to develop even to the perinatal…

Essential Role of Platelet-Derived: DISCUSSION(2)

According to Bruns et al. , a fully developed murine yolk sac can be morphologically divided into 5 regions: juxtauterine, periplacental, villous, smooth visceral, and in-traplacental yolk sac, the endodermal layers of which are derived from either parietal or visceral endoderm of the pre-gastrulating embryo. While PDGFRot is expressed in both endodermal layers of pre-gastrulating…

Essential Role of Platelet-Derived: DISCUSSION(1)

Recently, Bidwell et al. reported the localization of PDGFs and PDGF receptors in mouse extraembryonic tissues examined by immunohistochemistry and ISH, in which they concluded that PDGFRa is expressed in nucleated fetal erythrocytes, labyrinthine trophoblasts, and decidual cells. buy ampicillin

Essential Role of Platelet-Derived: RESULTS(4)

In these Pdgfraph/Pdgfraph mutants, macroscopically the placental disc, the yolk sac, and the vascular connections between the fetus and these organs appeared normal, except that the placental disc was smaller than those of normal littermates and the villous portion of the visceral yolk sac seemed to be less developed with fewer and shorter villi (Fig….

Essential Role of Platelet-Derived: RESULTS(3)

Expression of Calbindin-D9k in the Columnar Epithelial Cells of the IPY As previous studies have indicated that the expression of calbindin-D9k is the sole specific marker for the IPY in the mouse placental disc, we next examined whether or not ISH using antisense probe for our cloned putative mouse homologue of calbindin-D9k could detect the…

Essential Role of Platelet-Derived: RESULTS(2)

Although it was surmised that the IPY is an extension of the inverted yolk sac endoderm and its development commences before 14.5 dpc, little is known about the precise course of its development. We next examined the developmental course of this structure by tracing the changes in the expression pattern of PDGFRa in the placental…

Essential Role of Platelet-Derived: RESULTS(1)

PDGFRa Expression in the IPY Sectioned and sliced specimens of 14.5- or 15.5-dpc placental discs were immunostained with anti-PDGFRa monoclonal antibody, APA5. As shown in Figure 1, С and D, and Figure 2, К and L, strong APA5 staining was observed in the epithelial cells lining sinusoidal structures that extend to the chorioallantoic placenta and…

Essential Role of Platelet-Derived: MATERIALS AND METHODS(4)

As the result of the RT-PCR, an approximately 160-base pair (bp) DNA fragment was amplified from messenger RNA of 14.5-dpc ICR mouse placentas. This cDNA fragment was cloned into pCRII vector using a ТА cloning kit (Invitrogen), and its nucleotide sequence was determined using a DyeTerminator Cycle Sequencing system and ABI377 Autosequencer (Perkin-Elmer, Irvine, CA)….

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