Category Archives: Ovary

Activin, Inhibin, and Follistatin in Zebrafish Ovary: DISCUSSION(4)

The notion that activins and/or inhibins are local mediators of gonadotropin within the ovary is further supported by the finding that gonadotropin increased the expression of activin A- and inhibin A-like molecules. In vivo administration of hCG increased intensities of … Continue reading

Activin, Inhibin, and Follistatin in Zebrafish Ovary: DISCUSSION(3)

One of the most interesting findings from this study is that activin A and inhibin A had the same effect and did not antagonize each other in oocyte maturation. In agreement with these findings, an earlier study on goldfish pituitary … Continue reading

Activin, Inhibin, and Follistatin in Zebrafish Ovary: DISCUSSION(2)

The present study demonstrates that activin A and in-hibin A stimulate oocyte maturation in the zebrafish. Both activin A and inhibin A increased GVBD in a dose-dependent manner, with significant stimulation at 10-100 ng/ml. Although the physiological concentrations of activin … Continue reading

Activin, Inhibin, and Follistatin in Zebrafish Ovary: DISCUSSION(1)

Although the production of activins, inhibins, and FS by the ovary has been well documented in mammals, very little has been done in fish. The present study is the first one in fish to detect the intact molecules of activin, … Continue reading

Activin, Inhibin, and Follistatin in Zebrafish Ovary: RESULTS(3)

To determine the specificity of activin A and inhibin A action, the interaction between activin A and FS or inhibin A and FS was examined. Furthermore, the combined effect of activin A and inhibin A on oocyte maturation was also … Continue reading

Activin, Inhibin, and Follistatin in Zebrafish Ovary: RESULTS(2)

Activin A and Inhibin A Stimulated Zebrafish Oocyte Maturation Previous studies in the zebrafish have shown that only follicles that reach certain sizes can undergo maturation in vitro in response to exogenous hormones. To determine which sizes of follicles are … Continue reading

Activin, Inhibin, and Follistatin in Zebrafish Ovary: RESULTS(1)

Activin A-, Inhibin A-, and FS-Like Molecules Were Present in the Zebrafish Ovary To examine the expression of activin A, inhibin A and FS in the zebrafish ovary, RT-PCR was first performed using cDNA prepared from zebrafish ovary. A sense … Continue reading

Activin, Inhibin, and Follistatin in Zebrafish Ovary: MATERIALS AND METHODS(3)

In Vivo Injection Human CG (Sigma) was dissolved in 0.9% NaCl solution in a concentration of 20 IU/ml. Each fish received 50 ^l of saline (control) or hCG solution through i.m. injection. At 12 h, 24 h, and 30 h … Continue reading

Activin, Inhibin, and Follistatin in Zebrafish Ovary: MATERIALS AND METHODS(2)

Sequencing Analysis To confirm the identities of PCR products, DNA fragments were recovered from gels using Gene Clean II kit (Bio 101, Vista, CA) and were sequenced using PCR primers. All sequencing runs were performed using a DNA sequencer (Applied … Continue reading

Activin, Inhibin, and Follistatin in Zebrafish Ovary: MATERIALS AND METHODS(1)

Animals Adult zebrafish, Danio rerio, were obtained from a local aquarium and maintained in the laboratory according to the procedures of Westerfield. They were kept in filtered and aerated tanks at 28°C under a 14L:10D photoperiod. Experiments were performed according … Continue reading

Pages: 1 2 Next