Category Archives: Obese

Adipose Obese Gene Product: DISCUSSION(2)

Additionally, recent studies indicate that injections of leptin to ob/ ob mice, which have a congenital deficiency in leptin and are infertile, increase numbers of graafian follicles and correct their infertility . Although the positive effect of leptin on thecal … Continue reading

Adipose Obese Gene Product: DISCUSSION(1)

Results of the present studies have indicated that 1) leptin inhibits insulin-induced progesterone and androstenedione production by thecal cells; 2) leptin increases insulin-induced thecal cell numbers without affecting thecal cell viability; 3) leptin does not compete for [125I]insulin binding; and … Continue reading

Adipose Obese Gene Product: RESULTS

Experiment 1 In the absence of leptin, insulin increased (p < 0.05) thecal cell numbers (Fig. 1), and progesterone (Fig. 2A) and androstenedione (Fig. 2B) production to 1.2-, 2.6-, and 5.4-fold, respectively, of controls. Leptin at 10, 100, and 300 … Continue reading

Adipose Obese Gene Product: MATERIALS AND METHODS(4)

Experiment 4 was conducted to evaluate whether specific binding of 125I-leptin existed on thecal cells. Thecal cells from large follicles were cultured for 3 days in the presence of 10% FCS, and then cells were washed, and 125I-leptin binding assays … Continue reading

Adipose Obese Gene Product: MATERIALS AND METHODS(3)

Experiment 2 was conducted to evaluate whether the effect of leptin or insulin on thecal cell steroidogenesis was due to a change in cell viability. Thecal cells were cultured for 2 days in 10% FCS and then cultured in serum-free … Continue reading

Adipose Obese Gene Product: MATERIALS AND METHODS(2)

Medium was a 1:1 (v:v) mixture of DMEM and Ham’s F-12 containing 0.12 mM gentamicin and 38.5 mM sodium bicarbonate. Approximately 2 X 105 viable cells in 20-105 jxl of medium were added to Falcon multiwell plates (#3047; Becton Dickinson … Continue reading

Adipose Obese Gene Product: MATERIALS AND METHODS(1)

Reagents and Hormones Reagents were Dulbecco’s Modified Eagle’s Medium (DMEM), Ham’s F-12, insulin (bovine; 25.7 U/mg), enzymes, and fetal calf serum (FCS), all obtained from Sigma Chemical Company (St. Louis, MO); bovine LH (L1914, LH activity 2.0 X NIH-LH-S1 U/mg) … Continue reading

Adipose Obese Gene Product, Leptin, Inhibits Bovine Ovarian Thecal Cell Steroidogenesis

INTRODUCTION Leptin, the recently identified adipose obese (ob) gene product, is a plasma protein hormone that parallels the amount of fat reserves and is thought to regulate satiety. The initial 167-amino acid sequence of leptin is highly conserved (i.e., 83-97% … Continue reading

Adipose Obese Gene Product: DISCUSSION(3)

Results of the present study also suggest that ovarian thecal cells have high-affinity receptors for leptin. Comparison of the DNA sequence of the bovine leptin receptor with that of the human and mouse indicates a 81% and 75% sequence identity, … Continue reading