Category Archives: Myometrium - Part 2

Adenylyl Cyclase Messenger Ribonucleic Acid: MATERIALS AND METHODS(6)

Protein Assay Protein was assayed with the Pierce Chemical Co. (Rockford, IL) BCA protein assay reagent, consisting of a bicinchoninic acid and copper sulfate solution. BSA was used as a standard. buy ampicillin Isolation of RNA RNA was isolated using … Continue reading

Adenylyl Cyclase Messenger Ribonucleic Acid: MATERIALS AND METHODS(5)

Increased inositol phosphate formation in response to 1 ^M oxytocin was measured in confluent transformed myometrial cells grown in 24-well plates. Confluent cells were washed three times in serum-free and inositol-free DMEM medium and incubated overnight, with each well containing … Continue reading

Adenylyl Cyclase Messenger Ribonucleic Acid: MATERIALS AND METHODS(4)

To further characterize the phenotypic expression of cell surface receptors that are typical of myometrial cells, transformed cells were characterized for the expression of p-adrenergic and oxytocin receptors. The presence of p-ad-renergic receptors was confirmed by the measurement of adenylyl … Continue reading

Adenylyl Cyclase Messenger Ribonucleic Acid: MATERIALS AND METHODS(3)

Phenotypic Characterization of Transformed Cells Morphologically, immortalized cells demonstrate characteristics consistent with smooth muscle, including elongation to ends that fan out for broad areas of attachment. To confirm that these cells were of smooth muscle lineage, confluent cells were analyzed … Continue reading

Adenylyl Cyclase Messenger Ribonucleic Acid: MATERIALS AND METHODS(2)

For the establishment of cultured cells lines, myometrial cells were dispersed from dissected uteri by incubation for 90 min at 37°C in Hanks’ Balanced Salt Solution containing 640 ^g/ml collagenase type IV, 10 U/ml elastase type IV, 1 mg/ml soybean … Continue reading

Adenylyl Cyclase Messenger Ribonucleic Acid: MATERIALS AND METHODS(1)

Materials Hanks’ Balanced Salt Solution, Dulbecco’s Modified Eagle’s medium (DMEM), antibiotic-antimycotic solution, amphotericin B, Trizol, amplification-grade DNase I, random hexamer primers, Superscript I reverse transcriptase, RNase H, and Taq polymerase were purchased from Gibco Life Technologies (Gaithersburg, MD). Radioisotopes were … Continue reading

Adenylyl Cyclase Messenger Ribonucleic Acid in Myometrium: Splice Variant of Type IV

Regulation of uterine tone during pregnancy and parturition involves G protein-coupled receptors that modulate cellular levels of cAMP. The maintenance of uterine quiescence and the initiation of labor are influenced by hormonally induced changes in the expression of both a- … Continue reading

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