Category Archives: Luteal Regression - Part 2

Caspase-3 in the Rat Ovary: MATERIALS AND METHODS(3)

At the indicated times after serum, anti-eCG antibody, saline, or Lutalyse injection, ovaries were excised, cleared of adhering fat, and either fixed in 10% neutral buffered formalin for histology or placed in PBS containing protease inhibitors (10 mM PMSF, 10 … Continue reading

Caspase-3 in the Rat Ovary: MATERIALS AND METHODS(2)

Animal Treatments Immature female Sprague-Dawley rats were maintained on 12L:12D cycles, given food and water ad libitum, and killed by cervical dislocation. All procedures were carried out in accordance with the Guidelines for the Care and Use of Laboratory Animals, … Continue reading

Caspase-3 in the Rat Ovary: MATERIALS AND METHODS(1)

Materials Electrophoresis-grade Tris, acrylamide, A^iV-methylene-£/.v-acrylamide, glycine, dithiothreitol (DTT), SDS, ammonium persulfate, prestained low-range molecular weight markers, peroxidase-linked anti-rabbit IgG, peroxidase-linked anti-mouse IgG, nitrocellulose membranes, and a DC protein assay kit were purchased from Bio-Rad Laboratories (Richmond, CA). flovent inhaler

Caspase-3 in the Rat Ovary: Localization and Possible Role in Follicular Atresia and Luteal Regression(2)

These findings point to a central role for caspase-3 in apoptosis and demonstrate that caspase-3 activity results in the activation of the endogenous endonuclease. A limited number of substrates are cleaved by activated proteases during apoptosis including poly (ADP-ribose) polymerase … Continue reading

Caspase-3 in the Rat Ovary: Localization and Possible Role in Follicular Atresia and Luteal Regression(1)

Apoptosis is the cellular mechanism of ovarian follicular atresia and luteal regression. A common biochemical feature of apoptosis, the degradation of genomic DNA by the action of a Ca2+/Mg2+-dependent endonuclease, has been demonstrated in atretic follicles and regressing luteal tissue … Continue reading

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