Category Archives: Luteal Regression

Caspase-3 in the Rat Ovary: DISCUSSION(7)

Theca cells are the major site of LDL uptake and cholesterol synthesis in the follicle and are therefore likely to contain SREBP. However, the presence of this transcription factor in theca cells has not yet been reported. ventolin inhaler

Caspase-3 in the Rat Ovary: DISCUSSION(6)

SREBP is a membrane-bound transcription factor that is released and activated by proteases in response to low cellular cholesterol levels. The cleaved SREBP acts as a transcription factor to increase the synthesis of low-density lipoprotein (LDL) receptor, cholesterol-biosynthetic enzymes, and … Continue reading

Caspase-3 in the Rat Ovary: DISCUSSION(5)

For example, since steroidogenesis is dependent on the cy-toskeleton, actin degradation during apoptosis could account for the rapid loss of steroidogenesis associated with luteal regression. Functional regression of the bovine CL occurs before the onset of measurable DNA degradation, which … Continue reading

Caspase-3 in the Rat Ovary: DISCUSSION(4)

It is not yet known which, if any, of these substrates is cleaved to produce the key biochemical step in the initiation of apoptosis. Recently a novel heterodi-meric protein, DNA fragmentation factor (DFF), has been identified as a potential link … Continue reading

Caspase-3 in the Rat Ovary: DISCUSSION(3)

There are no reports of the requirement for caspase activity during luteal regression, but Rueda et al. demonstrated that caspase-1 mRNA is increased in the regressing bovine CL. Cleavage of actin and PARP during follicular atresia and luteal regression was … Continue reading

Caspase-3 in the Rat Ovary: DISCUSSION(2)

The localization of caspase-3 in the present studies does not distinguish between the proenzyme and the mature activated forms of the enzyme, but inhibitors of caspase-3 activity have been used to prevent apoptosis in granulosa cells, which suggests that the … Continue reading

Caspase-3 in the Rat Ovary: DISCUSSION(1)

The present study is the first to examine the localization of caspase-3 in the rat ovary and the changes in caspase-3 distribution during follicular atresia and luteal regression. buy flovent inhaler

Caspase-3 in the Rat Ovary: RESULTS(2)

Although TUNEL alone cannot distinguish between apoptosis and necrosis, we and others have previously demonstrated that apoptosis is the form of cell death prevalent during follicular atresia and luteal regression. For this reason, TUNEL-positive cells in ovarian sections were considered … Continue reading

Caspase-3 in the Rat Ovary: RESULTS(1)

Apoptosis was evident as an increase in the extent of DNA ladders in ovarian follicles treated with anti-eCG antiserum, or in luteal tissue from rats treated with PGF2a (Fig. 1). Actin cleavage to produce the 41-kDa product from the intact … Continue reading

Caspase-3 in the Rat Ovary: MATERIALS AND METHODS(4)

Western Analysis To prepare protein extracts, cell pellets were lysed by heating (95°C, 5 min) in SDS sample buffer (50 mM Tris [pH 7.5], 5% SDS, 5 mM DTT, 5% glycerol) containing 6 M urea and were cleared by centrifugation … Continue reading

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