Category Archives: Infertility

Association of the Mouse Infertility Factor: DISCUSSION(2)

While this report was in preparation, Maines and Was-serman reported that the Drosophila homologue of DAZL1—Boulc—controlledthetranslation ofTwine, a meiotic Cdc25-type phosphatase. They found that in boule mutants the translation, but not the transcription, of twine was significantly reduced, and that … Continue reading

Association of the Mouse Infertility Factor: DISCUSSION(1)

We present evidence for the association of DAZL1 with actively translating polyribosomes in testes. Our results suggest a role for DAZL1 in translational regulation of a subset of mRNAs in germ cells. Germ cell-specific translational regulation is well documented for … Continue reading

Association of the Mouse Infertility Factor: RESULTS(2)

Generation of Anti-DAZL1 Antibodies A polyclonal antiserum was generated against the last 76 amino acid residues of the human DAZL1 protein, which is 86% identical to the corresponding region of mouse DAZL1. On Western blots, the anti-DAZL1 antiserum detected a … Continue reading

Association of the Mouse Infertility Factor: RESULTS(1)

Binding of DAZ and DAZL1 to RNA Molecules The abilities of DAZ and DAZL1 to bind RNA molecules were studied using an assay in which in vitro-synthe-sized 35S-labeled proteins were incubated with RNA homopolymers immobilized on agarose beads, and the … Continue reading

Association of the Mouse Infertility Factor: MATERIALS AND METHODS(3)

Inhibition of Western Detection of DAZL1 Unlabeled DAZL1 was synthesized in vitro using the TNT Coupled Reticulocyte Lysate System (Promega). T3 and T7 RNA polymerase transcribed the Dazll cDNA clone in the sense and antisense directions, respectively. Varying amounts of … Continue reading

Association of the Mouse Infertility Factor: MATERIALS AND METHODS(2)

Generation of Deletion Constructs of DAZ and DAZL1 Deletion constructs of DAZL1 and DAZ were generated from cDNA clones DAZL1-f2 and DAZ-e11, respectively. Deletions were created either by using existing restriction sites within the coding regions or by polymerase chain … Continue reading

Association of the Mouse Infertility Factor: MATERIALS AND METHODS(1)

Generation of Anti-DAZL1 Antibody A 229-base pair Sau3AI fragment encoding the last 76 amino acid residues of DAZL1 was isolated from a human DAZL1 cDNA clone f2 and cloned into the BamHI site of an expression vector pET32b (Novagen, Madison, … Continue reading

Association of the Mouse Infertility Factor: INTRODUCTION

A requirement of the Y chromosome long arm (Yq) for normal spermatogenesis was recognized more than two decades ago when six infertile men were found to have Yq deletions. More recent molecular screening has detected Yq microdeletions in 10-15% of … Continue reading