Category Archives: Human Placenta

Gestational Regulation of Granulocyte-Colony Stimulating Factor Receptor Expression: DISCUSSION(4)

Undifferentiated cytotrophoblast cells of term fetal membranes also express G-CSFR yet do not have invasive properties. On the contrary, trophoblast cells in fetal membranes act as a physiological barrier between the fetus and the mother, and until parturition there is … Continue reading

Gestational Regulation of Granulocyte-Colony Stimulating Factor Receptor Expression: DISCUSSION(3)

The mechanisms for the control of G-CSFR expression in placental chorionic villi throughout gestation are unknown. In particular, there is a remarkably sharp drop in levels of G-CSFR protein between 13 and 14 wk gestation, suggesting that G-CSFR expression is … Continue reading

Gestational Regulation of Granulocyte-Colony Stimulating Factor Receptor Expression: DISCUSSION(2)

Western blotting analyses of G-CSFR in placental membrane indicated that placental G-CSFR protein expression is modulated according to gestational age and coincides with changes in G-CSFR mRNA levels. These data suggest that expression of G-CSFR at the materno-fetal interface may … Continue reading

Gestational Regulation of Granulocyte-Colony Stimulating Factor Receptor Expression: DISCUSSION(1)

In this study, we examined in detail the pattern of expression of G-CSFR mRNA and protein in placental chorionic villi and maternal decidua throughout gestation. We have shown that 1) expression of human placental mRNA and protein corresponding to membrane-bound … Continue reading

Gestational Regulation of Granulocyte-Colony Stimulating Factor Receptor Expression: RESULTS(4)

Expression of Placental G-CSFR Was Similar in Tissues from Patients Treated with Oxytocin, Prostaglandin E1 Analogues, or Mifepristone Detection of G-CSFR by immunohistochemistry and Western blotting, using mAb LM832, in the chorionic villi of first-trimester placentas from patients treated with … Continue reading

Gestational Regulation of Granulocyte-Colony Stimulating Factor Receptor Expression: RESULTS(3)

Western blotting of cell membrane protein preparations from first-, second-, and third-trimester placentas was performed to detect G-CSFR and actin (Fig. 4). Two distinct bands of 150 kDa and 120 kDa, which correspond to G-CSFR, could be detected with mAb … Continue reading

Gestational Regulation of Granulocyte-Colony Stimulating Factor Receptor Expression: RESULTS(2)

G-CSFR Was Expressed on Undifferentiated Cytotrophoblast Cells of Term Fetal Membranes The staining pattern obtained for G-CSFR and cytoker-atin in fetal membranes is shown in Figure 2. The tropho-blast cells in fetal membranes exhibited diffuse staining for G-CSFR, which, in … Continue reading

Gestational Regulation of Granulocyte-Colony Stimulating Factor Receptor Expression: RESULTS(1)

G-CSFR Was Expressed on the Cell Surface of Trophoblast Cells in the Maternal Decidua Immunohistochemical localization of G-CSFR in first-, second-, and third-trimester placental tissue is shown in Figure 1. Interstitial cytotrophoblast embedded in dense maternal connective tissue and decidual … Continue reading

Gestational Regulation of Granulocyte-Colony Stimulating Factor Receptor Expression: MATERIALS AND METHODS(3)

Total RNA was isolated from 11 first-trimester, 10 second-trimester, and 8 third-trimester placental tissues according to a standard procedure. Placental RNA (15 ^g) was subjected to RpA using the G-CSFR and GApDh probes and the HybSpeed RPA Kit (Ambion, Witney, … Continue reading

Gestational Regulation of Granulocyte-Colony Stimulating Factor Receptor Expression: MATERIALS AND METHODS(2)

Immunohistochemistry Immunohistochemical localization of G-CSFR, cytoker-atin 18, or mouse IgG was achieved by the use of monoclonal antibodies (mAbs) LM832 (G-CSFR) or JMB2 (cy-tokeratin 18), or mouse IgG, and the alkaline phosphatase-anti-alkaline phosphatase (APAAP) method as described previously.

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