Australian Regenerative Medicine Institute

Category: Growth Factor

Insulin-Like Growth Factor-2 Regulation of Conceptus Composition: DISCUSSION(6)

Later, the degeneration of Reichert’s membrane leaves the visceral yolk sac exposed as an extensive bounding barrier and fluid exchange may occur here, as well as at the placenta. The fluid content of the normal exocelomic plus amniotic cavities is influenced by conceptus genotype, and the volume is normally greatest at E16.5 : the volume…

Insulin-Like Growth Factor-2 Regulation of Conceptus Composition: DISCUSSION(5)

Deficiency of IGF-2 in the trophoblast decreased the mean fetal placenta weight by 21%, and deficiency in the ICM derivatives decreased it by 14% (Table 4). There was no significant change in the DNA content in either case (Table 5), and thus wet weight and DNA content give different indicators of size. With IGF-2 in…

Insulin-Like Growth Factor-2 Regulation of Conceptus Composition: DISCUSSION(4)

Placental wet weight is increased by IGF-2 excess, as seen in Igf2r ko mice, and decreased by IGF-2 deficiency, as observed in Igf2 ko mice and this study). The mouse placenta is an anatomical unit in which maternal decidual tissue and conceptus components make contact and intermix; thus the total placenta fraction had a dual…

Insulin-Like Growth Factor-2 Regulation of Conceptus Composition: DISCUSSION(3)

In the present small experimental series of litters, the following additional variables did not have significant effects: number of viable embryos in the litter, position in the uterus, or the size or Igf2 status of nearest neighbors. In each recombination series, the comparisons were between manipulated normal conceptuses (normal ICM + normal trophectoderm) and manipulated experimental…

Insulin-Like Growth Factor-2 Regulation of Conceptus Composition: DISCUSSION(2)

These early postimplantation interactions show that the growth of one part is dependent on the other. In addition, the growth of the whole conceptus is clearly subject to size regulation, because combined whole morulae develop into pups of normal size. buy cipro Later in development, it is likely that the size and functions of the placenta…

Insulin-Like Growth Factor-2 Regulation of Conceptus Composition: DISCUSSION(1)

IGF-2 and Growth of the Conceptus IGF-2 production by the conceptus begins to alter its size from E10.5. Substantial quantities of Igf2 mRNA can first be detected by autoradiography at E5.5 in trophectoderm derivatives, at E6.5-7.0 in the extra-embryonic endoderm and extra-embryonic mesoderm, and at E7.5 in the mesoderm of the embryo. Immunohistochemistry suggests that…

Insulin-Like Growth Factor-2 Regulation of Conceptus Composition: RESULTS(2)

Recombination Experiments The development of the recombined blastocysts to viable E16.5 conceptuses was recorded (Table 2). There was no evidence that an Igf2 ko component in either the trophec-toderm or the ICM derivatives influenced the success of development after the operation and transplantation. buy birth control online Further analysis was restricted to the 13 litters…

Insulin-Like Growth Factor-2 Regulation of Conceptus Composition: RESULTS(1)

In all experiments, the Igf2 null allele was transmitted by a heterozygous father, giving the genotypes Igf2 +m/-p (subsequently called Igf2ko) and Igf2 +m/+p (subsequently called ‘‘normal’’). ventolin 100 mcg Nonmanipulated Conceptuses To provide a baseline for the recombination experiments, observations were made on nonmanipulated conceptuses from 129J/Sv matings. The wet weight, dry weight, and…

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