Category Archives: Follicles

Distribution of the «1 to «6 Chains of Type IV Collagen: DISCUSSION(7)

The staining was not likely to be nonspecific, as Western immunoblot analyses of dissected follicles using the same antibodies produced bands only at the expected molecular weights for type IV collagen a chains, namely NC1 dimers (54 kDa) and monomers … Continue reading

Distribution of the «1 to «6 Chains of Type IV Collagen: DISCUSSION(6)

The thecal compartment of antral follicles has been shown by Northern analyses to express the a2 and a3 chains of type IV collagen and the laminin (31 and 71 chains, and our observations of the a1 and a2 chains of … Continue reading

Distribution of the «1 to «6 Chains of Type IV Collagen: DISCUSSION(5)

In the ovarian follicle, it is the granulosa cells that are present throughout follicular development associated with the follicular basal lamina. The theca, on the other hand, only differentiates in bovine follicles at approximately the time of antrum formation, well … Continue reading

Distribution of the «1 to «6 Chains of Type IV Collagen: DISCUSSION(4)

Thus there must be continued synthesis and substantial remodeling of the follicular basal lamina. Incorporation of a new segment into the follicular basal lamina would require that this network be broken, and segments of the basal lamina lacking type IV … Continue reading

Distribution of the «1 to «6 Chains of Type IV Collagen: DISCUSSION(3)

In the current study, follicular development was associated with a decrease in intensity or a complete loss of type IV collagen chains from the follicular basal lamina. Additionally, staining for a1(IV) and a2(IV) was frequently unevenly distributed along the follicular … Continue reading

Distribution of the «1 to «6 Chains of Type IV Collagen: DISCUSSION(2)

Here, we have demonstrated for the first time that the presence of individual type IV collagen a chains in the follicular basal lamina alters with follicular development. This is consistent with studies in other developing tissues. For example, during development … Continue reading

Distribution of the «1 to «6 Chains of Type IV Collagen: DISCUSSION(1)

This is the first study to localize any of the six a chains of type IV collagen within the follicular basal lamina of any species. The follicular basal lamina of primordial and small preantral follicles contained all of the a … Continue reading

Distribution of the «1 to «6 Chains of Type IV Collagen: RESULTS(5)

All of the rat monoclonal antibodies to type IV collagen a1 to a5 chains reacted well with bovine kidney (shown only for ai and a2; Fig. 4). The mouse monoclonal antibodies were not tested. Strong specific bands of approximately equal … Continue reading

Distribution of the «1 to «6 Chains of Type IV Collagen: RESULTS(4)

Immunostaining of the Thecal Vasculature The endothelial cell marker, factor VIII-related antigen, was colocalized with each of the type IV collagen a chains. In antral follicles, the arterioles and venules were located mainly at the extremities of the theca interna … Continue reading

Distribution of the «1 to «6 Chains of Type IV Collagen: RESULTS(3)

Type IV Collagen a3, a4, a5, and a6 in Antral Follicles Although the follicular basal laminae of some antral follicles stained for individual collagen chains (Fig. 3), this was not the case for all follicles; and the intensity of staining … Continue reading

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