Category Archives: Bovine Oocytes

Maintenance of Bovine Oocytes: DISCUSSION(4)

An explanation of these observations is that during the period of follicle culture, the oocytes are able to translate or posttranslationally modify essential proteins or to transcribe mRNAs essential for further embryonic development. In the present study, the percentage of … Continue reading

Maintenance of Bovine Oocytes: DISCUSSION(3)

When oocytes were cultured in intact antral follicles for various time periods, it was shown that shorter periods (24 and 48 h) resulted in significantly higher rates of GV arrest and subsequent maturation than for follicles maintained for longer periods. … Continue reading

Maintenance of Bovine Oocytes: DISCUSSION(2)

In this population of oocytes, okadaic acid, which is a potent inhibitor of protein phosphatases and accelerates GVBD in cattle oocytes, did not induce GVBD. There are a few reports regarding the quality of meiotically arrested oocytes and their subsequent … Continue reading

Maintenance of Bovine Oocytes: DISCUSSION(1)

The results of these studies on the role of follicle wall in the maintenance of the GV stage in bovine oocytes is supported by recent published data. In the present study, the percentage of oocytes that were maintained at the … Continue reading

Maintenance of Bovine Oocytes: RESULTS(3)

The oocyte group classified as intermediate includes all of the oocytes morphologically classified as between GV stage and MII, and those that had degenerated. Embryo Development after Antral Follicle Culture The development of oocytes cultured for 24 h within intact … Continue reading

Maintenance of Bovine Oocytes: RESULTS(2)

On prolonged culture of intact antral follicles, the percentages of the oocytes that remained at the GV stage after 24, 48, 96, and 168 hours were 96.8%, 89.1%, 28.5%, and 30.9%, respectively (Fig. 4). This gave a significant cubic decline … Continue reading

Maintenance of Bovine Oocytes: RESULTS(1)

Oocyte Maturation/Viability after Culture of Antral Follicles Immersed in Culture Medium All of the oocytes derived after culture of the antral follicles immersed in culture medium were maintained at GV stage, although in some of these oocytes, abnormal chromatin condensation … Continue reading

Maintenance of Bovine Oocytes: MATERIALS AND METHODS(9)

Experiment 4. In this experiment the effect of longer periods of antral follicle culture on the maintenance of mei-otic arrest was evaluated. In total, 367 antral follicles were cultured for 24 h (n = 105), 48 h (n = 82), … Continue reading

Maintenance of Bovine Oocytes: MATERIALS AND METHODS(8)

Matured oocytes were fixed and stained for evaluation of nuclear maturation to metaphase II (MII) stage. Experiment 2. This experiment was designed to evaluate the viability of oocytes derived from antral follicle culture in culture medium without the use of insert … Continue reading

Maintenance of Bovine Oocytes: MATERIALS AND METHODS(7)

In Vitro Embryo Culture At 46-48 h after coincubation of the spermatozoa and oocytes, cleaved embryos with at least 4 cells were selected, washed twice in a Hepes-buffered synthetic oviductal fluid (Hepes-SOF) medium (pH 7.4), and transferred to 20-^l droplets … Continue reading

Pages: 1 2 Next