Category Archives: Adrenal

Cortisol in Fetal Fluids and the Fetal Adrenal: DISCUSSION(3)

In eutherian mammals, interactions between the fetal hypothalamic-pituitary-adrenal (HPA) axis and the placenta stimulate the fetal adrenal at term, and results reported here provide evidence for a similar mechanism in the tammar wallaby. Production of PGE2 by the placenta at … Continue reading

Cortisol in Fetal Fluids and the Fetal Adrenal: DISCUSSION(2)

Exogenous glucocorticoid hormones induce premature birth in tammars accompanied by normal profiles of maternal hormones, so it is possible that fetal cortisol is the trigger for parturition in this marsupial. The tammar may be similar in this regard to species … Continue reading

Cortisol in Fetal Fluids and the Fetal Adrenal: DISCUSSION(1)

Cortisol concentrations in fetal fluids of the tammar wallaby increased dramatically over the last 7-8% of pregnancy. This pattern is found in all eutherian species that have been studied and is reported here for the first time in a marsupial. … Continue reading

Cortisol in Fetal Fluids and the Fetal Adrenal: RESULTS

Adrenal Cortisol Adrenal gland pairs from neonates weighed 1.56 ± 0.18 mg (n = 14) on the day of birth. The protein content of fetal and neonatal adrenals increased linearly from Day 24 pregnancy to Day 0 postpartum (Fig. 1a). Cortisol … Continue reading

Cortisol in Fetal Fluids and the Fetal Adrenal: MATERIALS AND METHODS(3)

The standard protocol was modified to increase assay sensitivity and to account for the different sample volumes and type. Additional standards were included, and all reagent volumes in the assay protocol were halved, except for the [125I]cortisol tracer, which was … Continue reading

Cortisol in Fetal Fluids and the Fetal Adrenal: MATERIALS AND METHODS(2)

Cortisol Production Adrenals from fetuses between Day 23 and Day 26 of gestation were placed in 24-well culture dishes in 1 ml of Medium 199 (Sigma, St. Louis, MO) and were sealed in an incubation chamber, gassed with 95% O2:5% … Continue reading

Cortisol in Fetal Fluids and the Fetal Adrenal: MATERIALS AND METHODS(1)

Animals Adult female tammars of Kangaroo Island (South Australia) origin were housed in open grassed enclosures, as previously described. Care and treatment of animals conformed to the Australian National Health and Medical Research Council guidelines, and all experiments were approved … Continue reading

Cortisol in Fetal Fluids and the Fetal Adrenal at Parturition in the Tammar Wallaby (Macropus eugenii )(2)

In humans, corticotropin-releasing factor produced at the end of pregnancy by the placenta appears to provide this stimulus. In both species, synthesis of adrenal trophic factors by the placenta is increased by cortisol, providing a positive feedback system that leads … Continue reading

Cortisol in Fetal Fluids and the Fetal Adrenal at Parturition in the Tammar Wallaby (Macropus eugenii )(1)

The mechanisms involved in initiating parturition in mammals are diverse (reviewed in ). However, maturation of the fetal adrenal and increased concentrations of the glucocorticoid cortisol in fetal blood during the last 4-8% of pregnancy have been reported in all … Continue reading